Journal: Advanced healthcare materials

Article Title: Designing Inherently Photodegradable Cell-Adhesive Hydrogels for 3D Cell Culture.

doi: 10.1002/adhm.202100632

Figure Lengend Snippet: Figure 2. A) Live/dead staining of normal human dermal fibroblasts (NHDF) with calcein-AM (green, live cells) and propidium iodide (red, dead cells) embedded in GelMA-free PEGMA/PEGDMA (Gel-N0) and GelMA-containing (Gel-N10) hydrogels on day 1, 7, 14 postencapsulation, followed by imaging with confocal microscopy (Leica TCS SPE, scale bar: 200 µm). B-i) NHDF embedded in Gel-N10 hydrogels on day 1 postencapsulation. Comparison of HepG2 cells encapsulated in (ii) Gel-N10 and (iii) Gel-N0 hydrogels (scale bar: 100 µm). C) Proliferation behavior of encapsulated HepG2 cells in Gel-N10 and Gel-N0 hydrogels over a period of 7 d assessed by PrestoBlue assay (n = 3). D) Toxicity evaluation of Gel-N10 photodegradation products by MTT proliferation assay with HeLa cells after 72 h exposure (n = 3). The concentration of nondiluted degradation products was 53 mg mL−1. Data are presented as mean ± SD and statistically evaluated by (C) Student’s t-test and (D) one-way ANOVA. *, **, *** represent p < 0.05, 0.01, and 0.001, respectively.

Article Snippet: Normal human dermal fibroblasts (NHDF), human liver cancer cells (HepG2), and HeLa cells were purchased from PromoCell GmbH (Heidelberg, Germany).

Techniques: Staining, Imaging, Confocal Microscopy, Comparison, Prestoblue Assay, Proliferation Assay, Concentration Assay

Journal: PLoS genetics

Article Title: Microenvironmental regulation by fibrillin-1.

doi: 10.1371/journal.pgen.1002425

Figure Lengend Snippet: Figure 6. Immunofluorescence analyses of WMS skin and fibroblast cultures. (a) Immunolocalization of ADAMTSL-6 on P16 wildtype, heterozygous and homozygous WMD littermates. Antibodies specific for ADAMTSL-6 [24] were used to stain littermate skin. As previously shown [24], ADAMTSL-6 staining was similar to fibrillin-1 immunostaining in wildtype skin. WMD mutant skin showed a reduction in ADAMTSL-6 immunostaining, indicating that deletion of the ADAMTSL binding site in fibrillin-1 prevents ADAMTSL-6 from binding to fibrillin-1 in vivo. (b) Human fibroblast cell cultures stained with antibodies to fibrillin-1 (pAb9543) after 4 days and 10 days of culture. Control (CRL2418) fibroblasts elaborated a typical fibrillin- 1 fibril matrix that was clearly abundant at day 4 and day 10. In contrast, WMS (5010) fibroblasts deposited abundant fibrillin-1 fibrils at day 4, but these fibrils were thinner and more diffuse. Even after 10 days in culture, the WMS fibroblasts failed to establish prominent bundles of fibrillin fibrils. (c) Fibroblast cultures from wildtype, heterozygous, and homozygous WMD mice stained with anti-fibrillin-1 (pAb9543) after 4 days in culture. Mutant fibroblast cultures elaborated typical abundant, long fibrillin-1 fibrils that appeared somewhat thicker than fibrils in wildtype cultures. Scale bars = 50 mm. doi:10.1371/journal.pgen.1002425.g006

Article Snippet: Cell cultures CRL2418, a normal dermal fibroblast cell line, was purchased from American Type Culture Collection.

Techniques: Immunofluorescence, Staining, Immunostaining, Mutagenesis, Binding Assay, In Vivo, Control

Journal: PLoS genetics

Article Title: Microenvironmental regulation by fibrillin-1.

doi: 10.1371/journal.pgen.1002425

Figure Lengend Snippet: Figure 7. Measurements of TGF-b in cultured fibroblasts; a-smooth muscle actin staining. (a) WMS fibroblasts (family members 5016 and 5010) secreted equal amounts of total TGF-b protein compared to controls (C1, C2, C3, and C4). (b) No significant differences were detected in amounts of active TGF-b present in the media of WMS fibroblasts compared to controls. Medium containing 10% fetal bovine serum was used to show baseline values. For experiments in (a) and (b), n = 2 or 3, and the error bars represent the standard deviation. (c) Skin from 6-month old

Article Snippet: Cell cultures CRL2418, a normal dermal fibroblast cell line, was purchased from American Type Culture Collection.

Techniques: Cell Culture, Staining, Standard Deviation